Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb347620

Description

Mouse IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Mouse IgG and Mouse Serum.
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

sheep anti-Mouse IgG Alkaline Phosphatase Conjugated Antibody, sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugation

Dot Blot of Sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.