Mouse IgG F(ab')2 Agarose Antibody

SKU: orb346278

Description

Mouse IgG F(ab')2 Agarose Antibody

Research Area

Molecular Biology

Images & Validation

• Tested Applications: IP
• Application Notes:
  Mouse IgG F(ab')2 Agarose is suitable for immunoprecipitation and Immunoaffinity purifications.

Key Properties

• Source: Mouse
• Biological Origin: Mouse
• Isotype: IgG F(ab')2
• Conjugation: Agarose
• Purity: This product is an purified Mouse IgG isolated from normal Mouse Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 mg of anti-Mouse IgG.

Storage & Handling

• Storage: Store vial at 4° C prior to opening. DO NOT FREEZE.
• Form/Appearance: Suspension of agarose beads
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mouse IgG F(ab')2 fragment Agarose Conjugate, Mouse IgG Fab2 Agarose

SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). MW: 5 µl Opal Prestained Marker. Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 2: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 3: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 4: Mouse IgM Kappa Myeloma Protein. Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.