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Catalog Number | orb753878 |
---|---|
Category | Proteins |
Description | Recombinant mouse IGF1 protein |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Tag | N-terminal His-IF2DI Tag |
Purity | Greater than 95% as determined by SDS-PAGE |
MW | 28.3 kDa |
Formula | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
UniProt ID | P05017 |
Source | E.Coli |
Expression System | 49-118 |
Expression Region | 49-118 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
Alternative names | IBP1, IGF I, IGF IA, IGF IB, IGF-I, Igf1, IGF1_HUM Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
81.7 kDa, 24.3 kDa | |
Mouse IGF-I R, His Tag (orb511248) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1). |
Unconjugated | |
90% | |
130.1 kDa | |
Mouse IGF-I R Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-mIGF1 is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
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