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Catalog Number | orb707215 |
---|---|
Category | Proteins |
Description | Recombinant Mouse IGF1 Protein is produced by E.coli expression system and the target gene encoding Gly49-Ala118 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IGF1 |
Entrez | 16000 |
UniProt ID | P05017 |
Protein Sequence | MFPAMPLSSL FVNGGPETLC GAELVDALQF VCGPRGFYFN KPTGYGSSIR RAPQTGIVDE CCFRSCDLRR LEMYCAPLKP TKAALEHHHH HH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0. |
Alternative names | IBP1 protein, Insulin-like growth factor I protein Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-mIGF1 is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
98.00% | |
12 KDa (reducing condition) |
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | |
Escherichia Coli |
> 95 % as determined by SDS-PAGE | |
7.7 kDa (predicted) |