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Mouse IGF1 protein
SKU: orb707215
Description
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E. coli |
|---|---|
| Reactivity | Mouse |
| Protein Sequence | MFPAMPLSSL FVNGGPETLC GAELVDALQF VCGPRGFYFN KPTGYGSSIR RAPQTGIVDE CCFRSCDLRR LEMYCAPLKP TKAALEHHHH HH |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0. |
| Disclaimer | For research use only |
Alternative Names
−IBP1; Insulin-like growth factor I; IGF-I; Mechano growth factor; MGF; Somatomedin-C
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 33.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-mIGF1 is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg Mouse IGF1 protein [orb753878]
ELISA, WB
Greater than 95% as determined by SDS-PAGE
28.3 kDa
E.Coli
50 μg, 200 μg, 100 μg, 1 mgRecombinantIGF-I,Mouse [orb1494838]
> 95% by SDS-PAGE analysis.
7.8 kDa, observed by reducing SDS-PAGE.
Escherichia coli.
50 μg, 10 μg, 1 mgMouse IGF1 Protein [orb80397]
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Escherichia Coli
1 mg, 10 μg, 50 μg
Quality Guarantee
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Mouse IGF1 protein (orb707215)
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