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| Catalog Number | orb782512 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Homocysteine(HCy) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Homocysteine(HCy). |
| Reactivity | Mouse |
| Concentration | 8000 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 8000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse HCy protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HCy. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HCy in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 125-8000 ng/mL |
| Sensitivity | 38.44 ng/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | Homocysteine ELISA Kit |
| Note | For research use only |
Mouse Homocysteine ELISA Kit [orb409064]
Mouse
4.7 nmol/mL-300 nmol/mL
1.1 nmol/mL
48 T, 10 x 96 T, 5 x 96 T, 96 T, 24 T
