Mouse HMGB-1 ELISA Kit Discontinued

SKU: orb775304

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1(HMG1).

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Standard: 3000 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 46.88-3000 pg/mL
• Sensitivity: 18.29 pg/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

HMGB1 ELISA Kit, HMG3 ELISA Kit, SBP1 ELISA Kit, Sulfoglucuronyl Carbohydrate Binding Protein ELISA Kit, Amphoterin ELISA Kit, High Mobility Group Box 1 Protein ELISA Kit

Quick Database Links

UniProt

P63158

Hui Qiu # 1 2, Zhiying Shao # 1, Xin Wen 1 2, Debao Qu 1 2, Zhengyang Liu 1, Ziqin Chen 1, Xinyan Zhang 1, Xin Ding 3 4, Longzhen Zhang HMGB1/TREM2 positive feedback loop drives the development of radioresistance and immune escape of glioblastoma by regulating TLR4/Akt signaling J Transl Med ., (2024)

PubMed: 39075517

Applications

ELISA

Reactivity

Mouse