Mouse Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ELISA Kit

• Catalog Number: orb775164
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor(HBEGF).
• Reactivity: Mouse
• Concentration: 1000 pg/mL
• UniProt ID: Q06186
• Tested applications: ELISA
• Application notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HBEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HBEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HBEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HBEGF in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.1 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: HB-EGF ELISA Kit, DTR ELISA Kit, DTS ELISA Kit, DT
• Research Area: Cancer Biology, Immunology & Inflammation, Infecti
• Note: For research use only
• Expiration Date: Please enquire.

References

Bingsheng Yang # 1 2 3, Jianwen Su # 1 2, Jichang Wu # 1 2, Zhongwen Wang 1 2, Jin Hu 1 2, Mankai Yang 1 2, Yihuang Lin 1 2, Mingchao Jin 1 2, Xiaochun Bai 4, Bin Yu 1 2, Xianrong Zhang 5 6 Macrophage-derived amphiregulin induces myofibroblast transition in adipogenic lineage precursors near Staphylococcus aureus abscess in bone marrow Nat Commun ., (2025)

PubMed: 40998791

Applications

ELISA

Reactivity

Mouse