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Mouse Hedgehog Homolog, Indian (IHH) ELISA Kit

Catalog Number: orb780418

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780418
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Homolog, Indian(IHH).
ReactivityMouse
Concentration10 ng/mL
UniProt IDP97812
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IHH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IHH in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.062 ng/mL
Sample TypesTissue homogenates, cell lysates and other biological fluids
Alternative namesBDA1 ELISA Kit, HHG2 ELISA Kit
NoteFor research use only
Mouse Hedgehog Homolog, Indian (IHH) ELISA Kit