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| Catalog Number | orb775360 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 40(HSP40). |
| Reactivity | Mouse |
| Concentration | 40 ng/mL |
| UniProt ID | Q9QYJ3 |
| Tested applications | ELISA |
| Application notes | standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSP40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSP40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSP40 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.63-40 ng/mL |
| Sensitivity | 0.242 ng/mL |
| Sample Types | Tissue homogenates, cell lysates and other biological fluids. |
| Alternative names | DnaJ ELISA Kit, DNAJB1 ELISA Kit, Sis1 ELISA Kit, Read more... |
| Note | For research use only |
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Mouse DNAJB1 (DnaJ homolog subfamily B member 1) ELISA Kit [orb2666944]
Mouse
0.156-10ng/ml
0.094ng/ml
96 T, 48 T
