Mouse Heat Shock 70kDa Protein 1A (HSPA1A) ELISA Kit

• Catalog Number: orb778254
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1A(HSPA1A).
• Tested applications: ELISA
• Reactivity: Mouse
• Range: 0.79-50 ng/mL
• Assay Time: 3.5h
• Concentration: 50 ng/mL
• UniProt ID: Q61696
• Sensitivity: 0.27 ng/mL
• Alternative names: HSP70-1 ELISA Kit, HSP-A1A ELISA Kit, HSPA1 ELISA
• Note: For research use only
• Application notes: standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSPA1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSPA1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSPA1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSPA1A in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Expiration Date: Please enquire.