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Mouse Growth Differentiation Factor 3 (GDF3) ELISA Kit

Catalog Number: orb780124

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780124
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 3(GDF3).
ReactivityMouse
Concentration500 pg/mL
UniProt IDQ07104
Tested applicationsELISA
Application notesstandard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GDF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GDF3 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range7.82-500 pg/mL
Sensitivity2.9 pg/mL
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Alternative namesVgr2 ELISA Kit, Vg-Related Gene 2 ELISA Kit
NoteFor research use only
Mouse Growth Differentiation Factor 3 (GDF3) ELISA Kit