Mouse Growth Associated Protein 43 (GAP43) ELISA Kit

SKU: orb777531

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Associated Protein 43(GAP43).

Research Area

Neuroscience, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAP43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAP43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAP43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAP43 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

F1 ELISA Kit, B-50 ELISA Kit, PP46 ELISA Kit, Neuron Growth-Associated Protein 43 ELISA Kit, Neuromodulin ELISA Kit, Axonal Membrane Protein GAP-43 ELISA Kit, Protein F1 ELISA Kit, Calmodulin-Binding Protein P-57 ELISA Kit, Neural Phosphoprotein B-50 ELISA Kit

Quick Database Links

UniProt

P06837