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Catalog Number | orb425869 |
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Category | Proteins |
Description | Recombinant of mouse GRO1/KC protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | The protein solution (1mg/ml) was lyophilized from 20mM phosphate buffer pH-7.4 and 0.1M NaCl. |
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Protein Sequence | APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK |
Application notes | Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard |
Source | Escherichia Coli |
Biological Activity | The biological activity was determined by measuring the ability to chemoattrat human neutrophilsat a concentration of 10ng/ml-100ng/ml. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized GRO1 Recombinant in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized KC Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Growth-regulated alpha protein, CXCL1, Platelet-de Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 90.0% as determined by SDS-PAGE. | |
Escherichia Coli |