Cart summary

You have no items in your shopping cart.

Mouse Glycine Dehydrogenase (GLDC) ELISA Kit

Catalog Number: orb780834

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780834
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase(GLDC).
Tested applicationsELISA
ReactivityMouse
Range0.16-10 ng/mL
Assay Time3.5h
Concentration10 ng/mL
UniProt IDQ91W43
Sensitivity0.064 ng/mL
Alternative namesGCSP ELISA Kit, NKH ELISA Kit, Decarboxylating ELI
Read more...
NoteFor research use only
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GLDC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GLDC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GLDC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GLDC in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesTissue homogenates, cell lysates and other biological fluids.
Expiration DatePlease enquire.
Mouse Glycine Dehydrogenase (GLDC) ELISA Kit