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Mouse Glutathione Peroxidase 1 (GPX1) ELISA Kit

Catalog Number: orb1947953

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1947953
CategoryAssays and Kits
DescriptionMouse Glutathione Peroxidase 1 (GPX1) ELISA Kit
ReactivityMouse
Range62.5-4000pg/mL
Assay Time3.5H
TargetGPX1
UniProt IDP11352
Sensitivity37.5 pg/mL
Alternative namesGSHPx, GSH-Px
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GPX1. You can calculate the concentration of Mouse GPX1 in the samples by comparing the OD of the samples to the standard curve.
Sample TypesTissue homogenate and Cell lysate
Expiration DatePlease enquire.
Mouse Glutathione Peroxidase 1 (GPX1) ELISA Kit