Mouse Glutaminase 2 (GLS2) ELISA Kit

SKU: orb780047

Description

The Mouse Glutaminase 2 (GLS2) ELISA Kit uses a sandwich enzyme immunoassay to quantify Glutaminase 2 (GLS2) in mouse serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 1.57-100 ng/mL and a sensitivity of 0.53 ng/mL.

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GLS2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GLS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GLS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GLS2 in the samples is then determined by comparing the OD of the samples to the standard curve

  Read more...

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.53 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

GA ELISA Kit, GLS ELISA Kit, LGA ELISA Kit, hLGA ELISA Kit, L-glutaminase ELISA Kit, L-glutamine amidohydrolase ELISA Kit

Quick Database Links

UniProt

Q571F8