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Mouse Ghrelin (GHRL) ELISA Kit

Catalog Number: orb775809

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb775809
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ghrelin(GHRL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ghrelin(GHRL).
Tested applicationsELISA
ReactivityMouse
Range156.25-10000 pg/mL
Assay Time2.5h
Concentration10000 pg/mL
UniProt IDQ9UBU5
Sensitivity45.6 pg/mL
Alternative namesMTLRP ELISA Kit, Growth Hormone-Releasing Peptide
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NoteFor research use only
Application notesstandard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GHRL in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Mouse Ghrelin (GHRL) ELISA Kit

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