Mouse Gelsolin (GS) ELISA Kit

SKU: orb2667842

Description

Mouse Gelsolin (GS) ELISA Kit

Images & Validation

• Application Notes:
  standard: 500 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GS in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 µg/mL
• Sensitivity: 2.2 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GSN; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

Quick Database Links

UniProt

P13020