Mouse FTH ELISA Kit Discontinued

SKU: orb780248

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin, Heavy Polypeptide(FTH).

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Standard: 10 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FTH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 500 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

FTH1 ELISA Kit, FTHL6 ELISA Kit, PIG15 ELISA Kit, PLIF ELISA Kit, Ferritin Heavy Chain ELISA Kit, Apoferritin ELISA Kit, Placenta Immunoregulatory Factor ELISA Kit, Proliferation-Inducing Protein 15 ELISA Kit, Cell proliferation-inducing gene 15 protein ELISA Kit

Quick Database Links

UniProt

P09528

Di Xie 1, Yanli Ma 2, Chengjin Gao 3, Shuming Pan Piezo1 activation on microglial cells exacerbates demyelination in sepsis by influencing the CCL25/GRP78 pathway Int Immunopharmacol, (2024)

PubMed: 39236454

Applications

ELISA

Reactivity

Mouse