You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1950011 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse fT3 antigen, and the Mouse fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse fT3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Mouse |
Range | 0.32-20 ng/mL |
Assay Time | 1.5h |
Concentration | 20 ng/mL |
Sensitivity | 0.31 ng/mL |
Alternative names | 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Read more... |
Note | For research use only |
Application notes | standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse fT3 antigen, and the Mouse fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse fT3 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma |
Expiration Date | Please enquire. |