Mouse Free Triiodothyronine (fT3) EasyStep ELISA Kit

• Catalog Number: orb1950011
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse fT3 antigen, and the Mouse fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Mouse
• Concentration: 20 ng/mL
• Application notes: standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse fT3 antigen, and the Mouse fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse fT3 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 1.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.31 ng/mL
• Sample Types: Serum, plasma
• Alternative names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel;
• Note: For research use only