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Mouse Free Triiodothyronine (fT3) EasyStep ELISA Kit

Mouse Free Triiodothyronine (fT3) EasyStep ELISA Kit

Catalog Number: orb1950011

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb1950011
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse fT3 antigen, and the Mouse fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
ReactivityMouse
Range0.32-20 ng/mL
Assay Time1.5h
Concentration20 ng/mL
Sensitivity0.31 ng/mL
Alternative names3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel;
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NoteFor research use only
Application notesstandard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse fT3 antigen, and the Mouse fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse fT3 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma
Expiration DatePlease enquire.