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Mouse Fibrinogen Like Protein 1 (FGL1) ELISA Kit

Catalog Number: orb780498

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780498
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Like Protein 1(FGL1).
Tested applicationsELISA
ReactivityMouse
Range3.13-200 ng/mL
Assay Time3.5h
Concentration200 ng/mL
UniProt IDQ71KU9
Sensitivity1.35 ng/mL
Alternative namesFGL-1 ELISA Kit, HFREP1 ELISA Kit, HP-041 ELISA Ki
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NoteFor research use only
Application notesstandard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FGL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FGL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FGL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FGL1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Mouse Fibrinogen Like Protein 1 (FGL1) ELISA Kit