Mouse Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

SKU: orb1496498

Description

Mouse ERK2 ELISA Kit

Research Area

Cancer Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ERK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ERK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ERK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ERK2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 13.6 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MAPK1; P38; P40; ERK2; ERT1; MAPK2; P42MAPK; P42-MAPK; PRKM1; PRKM2; P41; P41mapk; P41-mapk; Mitogen-Activated Protein Kinase 1; Mitogen-activated protein kinase 2; ERK1/2

Quick Database Links

UniProt

P63085