Recombinant Mouse EGF Protein

SKU: orb3002397

Description

Recombinant Mouse Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn977-Arg1029 is expressed with a 6His tag at the C-terminus.

Research Area

Cancer Biology

Key Properties

• Expression System: E.coli
• Biological Activity: Cell Based Assay: Measured by its ability to inhibits the proliferation of human epithelial A431 cells.The ED50 for this effect is 2-20 ng/ml (QC verified). Human endometrial epithelial organoids were cultured with EGF (Cat#orb3002397) and R-spondin 1 (Cat#orb3002497). The organoids showed good morphology. Data were kindly provided by Novoprotein's customer. Thanks for her contribution.
• Tag: C-6His
• Expression Region: Asn977-Arg1029
• Endotoxins: < 1 EU/µg as determined by LAL test.
• MW: Predicted: 7.2 KDa. Observed: 9-14 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pro-epidermal growth factor; Epidermal growth factor; EGF

Quick Database Links

UniProt

P01132