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Mouse Doublecortin Like Kinase 1 (DCLK1) ELISA Kit

Catalog Number: orb1949811

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1949811
CategoryAssays and Kits
DescriptionMouse Doublecortin Like Kinase 1 (DCLK1) ELISA Kit
ReactivityMouse
Range0.16-10ng/mL
Assay Time3.5H
TargetDCLK1
UniProt IDQ9JLM8
Sensitivity0.1 ng/mL
Alternative namesCL1, CLICK1, DCAMKL1, DCDC3A, DCLK
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DCLK1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DCLK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DCLK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse DCLK1. You can calculate the concentration of Mouse DCLK1 in the samples by comparing the OD of the samples to the standard curve.
Sample TypesTissue homogenate
Expiration DatePlease enquire.
Mouse Doublecortin Like Kinase 1 (DCLK1) ELISA Kit