Mouse Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit

• Catalog Number: orb1088178
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 1A(CDKN1A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 1A(CDKN1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase Inhibitor 1A(CDKN1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase Inhibitor 1A(CDKN1A) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Mouse
• Concentration: 10 ng/mL
• UniProt ID: P39689
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CDKN1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CDKN1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CDKN1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CDKN1A in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.062 ng/mL
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Alternative names: WAF1, p21, CIP1, p21WAF1, CAP20, CDKN1, MDA-6, SDI
• Note: For research use only