Mouse Cyclic AMP Response Element Binding Protein (CREB) ELISA Kit

• Catalog Number: orb2668143
• Category: Assays and Kits
• Description: Mouse Cyclic AMP Response Element Binding Protein (CREB) ELISA Kit
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• UniProt ID: Q01147
• Sensitivity: 0.049 ng/mL
• Alternative names: CREB; active transcription factor CREB; cAMP respo
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CREB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CREB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CREB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CREB in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Tissue homogenates and other biological fluids.
• Expiration Date: Please enquire.