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Catalog Number | orb775712 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cross Linked N-Telopeptide Of Type I Collagen(NTXI) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cross Linked N-Telopeptide Of Type I Collagen(NTXI). |
Tested applications | ELISA |
Reactivity | Mouse |
Range | 3.13-200 ng/mL |
Assay Time | 2.5h |
Concentration | 200 ng/mL |
Sensitivity | 0.91 ng/mL |
Alternative names | NTX-I ELISA Kit, NTX1 ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NTXI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NTXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NTXI in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, urine and other biological fluids |
Expiration Date | Please enquire. |
Mouse | |
3.125-200ng/ml | |
1.875ng/ml |