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Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

Catalog Number: orb2667867

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667867
CategoryAssays and Kits
DescriptionMouse Connective Tissue Growth Factor (CTGF) ELISA Kit
UniProt IDP29268
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CTGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CTGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CTGF in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.11 ng/mL
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesCCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Fac
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NoteFor research use only
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