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Mouse Complement Component 5a Receptor 1 (C5aR1) ELISA Kit

Catalog Number: orb778107

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb778107
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5a Receptor 1(C5aR1).
Tested applicationsELISA
ReactivityMouse
Range0.32-20 ng/mL
Assay Time3.5h
Concentration20 ng/mL
UniProt IDP30993
Sensitivity0.113 ng/mL
Alternative namesCD88 ELISA Kit, C5A ELISA Kit, C5-AR1 ELISA Kit, C
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NoteFor research use only
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C5aR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C5aR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C5aR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C5aR1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typestissue homogenates, cell lysates and other biological fluids
Expiration DatePlease enquire.
Mouse Complement Component 5a Receptor 1 (C5aR1) ELISA Kit