Mouse Complement Component 3 (C3) ELISA Kit

• Catalog Number: orb2667253
• Category: Assays and Kits
• Description: Mouse Complement Component 3 (C3) ELISA Kit
• UniProt ID: P01027
• Application notes: standard: 500 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C3 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.81-500 µg/mL
• Sensitivity: 2.13 µg/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: ASP; CPAMD1; Complement Protein C3; C3 and PZP-lik
• Research Area: Immunology & Inflammation, Infectious Disease & Vi
• Note: For research use only
• Expiration Date: Please enquire.