Mouse Complement C5 Protein, His Tag

SKU: orb653662

Description

Mouse Complement C5, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 1680 (Accession # P06684-1).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (β chain) and 114.6 kDa (α chain). The protein migrates as 66 kDa (β chain) and 100 kDa (α chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: Complement C5
• Reactivity: Mouse
• Tag: C-10×His
• Molecular Weight: 73.5 kDa (β chain) & 114.6 kDa (α chain)
• Expression Region: Gln 19 - Leu 678 & Asn 679 - Glu 1680
• Purity: 95%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Mouse Complement C5, His Tag (Cat. No. orb653662) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-13 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5

UniProt

P06684

RefSeq:P06684