Mouse Coagulation Factor IX (F9) ELISA Kit

SKU: orb775426

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor IX(F9).

Research Area

Hematology, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Plasma
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.66 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

HEMB ELISA Kit, FIX ELISA Kit, GLA Domain ELISA Kit, PTC ELISA Kit, Anti Hemophilic Factor B ELISA Kit, Christmas Factor ELISA Kit, Plasma Thromboplastic Component ELISA Kit, Christmas Disease ELISA Kit, Hemophilia B ELISA Kit

Quick Database Links

UniProt

P16294