Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit

SKU: orb780163

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromatin Assembly Factor 1, Subunit B(CHAF1B).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CHAF1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CHAF1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CHAF1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CHAF1B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Tissue homogenates, cell Lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.053 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CAF-1 ELISA Kit, CAF-IP60 ELISA Kit, CAF1 ELISA Kit, CAF1A ELISA Kit, CAF1P60 ELISA Kit, MPHOSPH7 ELISA Kit, MPP7 ELISA Kit, M-Phase Phosphoprotein 7 ELISA Kit, Chromatin Assembly Factor I,p60 subunit ELISA Kit, M-phase phosphoprotein 7 ELISA Kit

Quick Database Links

UniProt

Q9D0N7