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Mouse Chordin Like Protein 2 (CHRDL2) ELISA Kit

Catalog Number: orb780627

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780627
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chordin Like Protein 2(CHRDL2).
ReactivityMouse
Concentration10 ng/mL
UniProt IDQ8VEA6
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CHRDL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CHRDL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CHRDL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CHRDL2 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.06 ng/mL
Sample TypesTissue homogenates, cell lysates and other biological fluids
Alternative namesBNF1 ELISA Kit, CHL2 ELISA Kit, Breast Tumor Novel
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NoteFor research use only
Mouse Chordin Like Protein 2 (CHRDL2) ELISA Kit