Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

• Catalog Number: orb774888
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Ligand 1(CX3CL1).
• Reactivity: Mouse
• Concentration: 10 ng/mL
• UniProt ID: O35188
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CX3CL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CX3CL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CX3CL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CX3CL1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: NTN ELISA Kit, ABCD3 ELISA Kit, C3Xkine ELISA Kit,
• Note: For research use only