Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

SKU: orb774888

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Ligand 1(CX3CL1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CX3CL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CX3CL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CX3CL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CX3CL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NTN ELISA Kit, ABCD3 ELISA Kit, C3Xkine ELISA Kit, CXC3 ELISA Kit, CXC3C ELISA Kit, NTT ELISA Kit, SCYD1 ELISA Kit, ABCD3 ELISA Kit, FKN ELISA Kit, Neurotactin ELISA Kit, Fractalkine ELISA Kit, Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1 ELISA Kit

Quick Database Links

UniProt

O35188