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Mouse Cathepsin G (CTSG) ELISA Kit

Mouse Cathepsin G (CTSG) ELISA Kit

Catalog Number: orb2667579

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667579
CategoryAssays and Kits
DescriptionMouse Cathepsin G (CTSG) ELISA Kit
Range0.78-50 ng/mL
Assay Time3.5h
UniProt IDP28293
Sensitivity0.21 ng/mL
Alternative namesCG; CTS-G
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NoteFor research use only
Application notesstandard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CTSG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CTSG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CTSG in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.