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Mouse CA12 protein

SKU: orb707090

Description

Recombinant Mouse Carbonic Anhydrase 12 Protein is produced by mammalian expression system and the target gene encoding Ala25-Ser301 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
TargetCA12
ReactivityMouse
Protein SequenceAPLNGSKWTY VGPAGEKNWS KKYPSCGGLL QSPIDLHSDI LQYDASLAPL QFQGYNVSVE KLLNLTNDGH SVRLNLNSDM YIQGLQPHHY RAEQLHLHWG NRNDPHGSEH TVSGKHFAAE LHIVHYNSDL YPDFSTASDK SEGLAVLAVL IEIGSANPSY DKIFSHLQHV KYKGQQVLIP GFNIEELLPE SPGEYYRYEG SLTTPPCYPT VLWTVFRNPV QISQEQLLAL ETALYFTHMD DPTPREMINN FRQVQKFDER LVYISFRQGL LTDTGLSVDH HHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 7.5.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Mouse CA12 protein (orb707090)

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Available Sizes

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10 μg
$ 380.00
50 μg
$ 1,000.00
500 μg
$ 4,850.00
DispatchUsually dispatched within 5-10 working days
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