Mouse Butyrylcholinesterase (BCHE) ELISA Kit

SKU: orb781643

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Butyrylcholinesterase(BCHE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Butyrylcholinesterase(BCHE).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse BCHE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCHE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BCHE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 0.88 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BuChE ELISA Kit, CHE1 ELISA Kit, E1 ELISA Kit, Pseudocholinesterase ELISA Kit, Cholinesterase ELISA Kit, Acylcholine acylhydrolase ELISA Kit, Butyrylcholine esterase ELISA Kit, Choline esterase II ELISA Kit