Mouse BMP3(Bone Morphogenetic Protein 3) Microsample ELISA Kit

SKU: orb2999463

Description

The Mouse BMP3(Bone Morphogenetic Protein 3) Microsample ELISA Kit is designed to quantify BMP3(Bone Morphogenetic Protein 3) Microsample in mouse serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 31.25-2000 pg/mL and a sensitivity of 14 pg/mL.

Research Area

Stem Cell & Developmental Biology; Musculoskeletal & Connective Tissue Research

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BMP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BMP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 14 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BMP3A; BMP-3A; Osteogenic

Quick Database Links

UniProt

Q8BHE5