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| Catalog Number | orb780339 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biglycan(BGN). |
| Tested applications | ELISA |
| Reactivity | Mouse |
| Range | 3.13-200 ng/mL |
| Assay Time | 3.5h |
| Concentration | 200 ng/mL |
| UniProt ID | P28653 |
| Sensitivity | 1.41 ng/mL |
| Alternative names | DSPG1 ELISA Kit, PG-S1 ELISA Kit, PGI ELISA Kit, S Read more... |
| Note | For research use only |
| Application notes | standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BGN in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
Mouse BGN ELISA Kit [orb407805]
Mouse
0.625 ng/mL-40 ng/mL
0.156 ng/mL
24 T, 96 T, 10 x 96 T, 5 x 96 T, 48 T
