Mouse B-Cell Activating Factor (BAFF/CD257) ELISA Kit

• Catalog Number: orb2667790
• Category: Assays and Kits
• Description: Mouse B-Cell Activating Factor (BAFF/CD257) ELISA Kit
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• UniProt ID: Q9WU72
• Sensitivity: 0.067 ng/mL
• Alternative names: CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.