Mouse Arginine Vasopressin Receptor 2 (AVPR2) ELISA Kit

SKU: orb781076

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin Receptor 2(AVPR2).

Research Area

Cardiovascular Research, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AVPR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AVPR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AVPR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AVPR2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.122 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

ADHR ELISA Kit, DI1 ELISA Kit, DIR ELISA Kit, DIR3 ELISA Kit, NDI ELISA Kit, V2R ELISA Kit, Nephrogenic Diabetes Insipidus ELISA Kit, Vasopressin V2 receptor ELISA Kit, Antidiuretic hormone receptor ELISA Kit, Renal-type arginine vasopressin receptor ELISA Kit

Quick Database Links

UniProt

O88721