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Mouse Apelin (APLN) ELISA Kit

Catalog Number: orb780855

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb780855
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin(APLN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin(APLN).
ReactivityMouse
Concentration8000 pg/mL
UniProt IDQ9R0R4
Tested applicationsELISA
Application notesstandard: 8000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse APLN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeCompetitive
Assay Time2.5h
Range125-8000 pg/mL
Sensitivity34.8 pg/mL
Sample TypesSerum, plasma and other biological fluids.
Alternative namesXNPEP2 ELISA Kit, APEL ELISA Kit, APJ endogenous l
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NoteFor research use only
Mouse Apelin (APLN) ELISA Kit

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    125-8000pg/mL

    37.5pg/mL

    48 T, 96 T