Mouse Antidiuretic Hormone (ADH) ELISA Kit

SKU: orb777573

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Antidiuretic Hormone(ADH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Antidiuretic Hormone(ADH).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse ADH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ADH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ADH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.22 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ARVP ELISA Kit, AVP ELISA Kit, VP ELISA Kit, Arginine Vasopressin ELISA Kit, Arg-Vasopressin ELISA Kit, Antidiuretic Hormone ELISA Kit, Diabetes Insipidus ELISA Kit, Neurohypophyseal ELISA Kit, Argipressin ELISA Kit