Mouse Anti-SARS-CoV2 (S-RBD) (Omicron, B.1.1.529) IgG ELISA Kit

• Catalog Number: orb1496480
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S-BRD IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S-BRD IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S-BRD IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S-BRD IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Mouse
• Range: 1.57-100 ng/mL
• Assay Time: 3.5h
• Concentration: 100 ng/mL
• Sensitivity: 0.91 ng/mL
• Alternative names: anti-COVID-19;anti-SARS-CoV-2; anti-2019-nCoV
• Note: For research use only
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S-BRD IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S-BRD IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S-BRD IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S-BRD IgG in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma and biological fluids
• Expiration Date: Please enquire.