Mouse Anti-Cardiolipin Antibody IgG (ACA-IgG) ELISA Kit

• Catalog Number: orb1291273
• Category: Assays and Kits
• Description: Mouse ACA-IgG (Anti-Cardiolipin Antibody IgG) ELISA Kit
• Reactivity: Mouse
• Concentration: 200 ng/mL
• Tested applications: ELISA
• Application notes: standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACA-IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACA-IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACA-IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACA-IgG in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.028 ng/mL
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Alternative names: ACA-IgG, Anti-Cardiolipin Antibody IgG
• Research Area: Cardiovascular Research
• Note: For research use only
• Expiration Date: Please enquire.