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Mouse Anti-Cardiolipin Antibody IgG (ACA-IgG) ELISA Kit

Catalog Number: orb1291273

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1291273
CategoryAssays and Kits
DescriptionMouse ACA-IgG (Anti-Cardiolipin Antibody IgG) ELISA Kit
Tested applicationsELISA
ReactivityMouse
Range3.13-200 ng/mL
Assay Time3.5h
Concentration200 ng/mL
Sensitivity1.028 ng/mL
Alternative namesACA-IgG, Anti-Cardiolipin Antibody IgG
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NoteFor research use only
Application notesstandard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACA-IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACA-IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACA-IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACA-IgG in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.
Mouse Anti-Cardiolipin Antibody IgG (ACA-IgG) ELISA Kit