Mouse Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit

SKU: orb780465

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amine Oxidase, Copper Containing 3(AOC3).

Research Area

Infectious Disease & Virology, Metabolism Research, Musculoskeletal & Connective Tissue Research, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AOC3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AOC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AOC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AOC3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.67 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

VAP1 ELISA Kit, HPAO ELISA Kit, SSAO ELISA Kit, Membrane primary amine oxidase ELISA Kit, Vascular Adhesion Protein 1 ELISA Kit, Semicarbazide-Sensitive Amine Oxidase ELISA Kit, Copper-Containing Amine Oxygen Oxidoreductase ELISA Kit

Quick Database Links

UniProt

O70423