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Mouse Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit

Catalog Number: orb780465

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780465
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amine Oxidase, Copper Containing 3(AOC3).
Tested applicationsELISA
ReactivityMouse
Range1.57-100 ng/mL
Assay Time3.5h
Concentration100 ng/mL
UniProt IDO70423
Sensitivity0.67 ng/mL
Alternative namesVAP1 ELISA Kit, HPAO ELISA Kit, SSAO ELISA Kit, Me
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NoteFor research use only
Application notesstandard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AOC3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AOC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AOC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AOC3 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.
Mouse Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit