Mouse Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit

• Catalog Number: orb779396
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-1-Microglobulin/Bikunin Precursor(a1M) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Microglobulin/Bikunin Precursor(a1M).
• Reactivity: Mouse
• Concentration: 200 µg/mL
• Tested applications: ELISA
• Application notes: standard: 200 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse a1M protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1M in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 2.5h
• Range: 3.13-200 µg/mL
• Sensitivity: 0.97 µg/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: AMBP ELISA Kit, UTI ELISA Kit, HCP ELISA Kit, EDC1
• Note: For research use only