Mouse Alpha-1-Antichymotrypsin (a1ACT) ELISA Kit

SKU: orb2667140

Description

Mouse Alpha-1-Antichymotrypsin (a1ACT) ELISA Kit

Research Area

Gastroenterology & Hepatology, Infectious Disease & Virology, Neuroscience, Protein Biochemistry

Images & Validation

• Application Notes:
  standard: 1000 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse a1ACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1ACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse a1ACT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1ACT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 µg/mL
• Sensitivity: 5.25 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A1-ACT; SERPINA3; AACT; ACT; GIG24; GIG25; Serpin Peptidase Inhibitor Clade A Member 3(Alpha-1 Antiproteinase; Antitrypsin); Cell growth-inhibiting gene 24/25 protein