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Mouse Agouti Related Protein (AGRP) ELISA Kit

Catalog Number: orb777628

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb777628
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Agouti Related Protein(AGRP).
ReactivityMouse
Concentration2000 pg/mL
UniProt IDP56473
Tested applicationsELISA
Application notesstandard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AGRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AGRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AGRP in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range31.25-2000 pg/mL
Sensitivity12 pg/mL
Sample Typesserum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Alternative namesAGRT ELISA Kit, ASIP2 ELISA Kit, ART ELISA Kit, Ag
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NoteFor research use only
Mouse Agouti Related Protein (AGRP) ELISA Kit